Figure 1
From: EST Express: PHP/MySQL based automated annotation of ESTs from expression libraries
Data Pipeline. Raw sequence data is imported into EST Express along with phred scores, where it is then screened for contaminating vector sequences and masked for quality. Good quality sequences are then batch BLASTed against a local UniGene database and the top hit is assigned to each sample. A local copy of the Entrez Gene database is then linked to the UniGene identifier and used to annotate each sequence with a description, Gene Ontology identifiers, RefSeq mRNA and protein links, and genomic context. Oligo(dT)-primed sequences can then be analyzed for full-length status using a local copy of the RefSeq protein database and the Entrez Gene cross references. The user interface then provides several ways to browse and visualize the results from the pipeline.