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Table 3 Sequencing success rates for projects by amplification protocol and region type

From: Novel computational methods for increasing PCR primer design effectiveness in directed sequencing

 

Standard

High GC

Combined Amplicon Statistics

Projects

# Amp.

% Amp. Success

# Amp.

% Amp. Success

# Total Amp.

% Amp. Success

# Exonic Amp.

% Exonic Amp. Success

JCVI #1

3,529

94.87%

1,786

82.42%

5,315

90.69%

1,680

94.52%

JCVI #2

465

93.98%

282

98.94%

747

95.85%

230

96.96%

JCVI #3

12

83.33%

28

100.00%

40

95.00%

10

100.00%

JCVI #4

10

100.00%

10

100.00%

20

100.00%

15

100.00%

JCVI #5

422

95.73%

427

90.16%

849

92.93%

777

96.14%

Total

4,438

94.84%

2,533

85.83%

6,971

91.57%

2,712

95.24%