Figure 1
From: Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry
Effect of different f-parameter values on a fitted qPCR curve. A five parameter log-logistic model was fitted on the real-time PCR curve of the S27a transcript (black line, fit; black circles, experimental measurements). The effect of varying the f-parameter between 1.0 and 5.0 while keeping the other parameters constant is displayed as coloured curves. Altering the f-parameter also influences other parameters of the fit, mainly 'b' (the slope) and 'e' (the inflection point), although the latter is in a five parameter model different from the classical definition (see Discussion).