Figure 2
From: Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry
Scenario A: qPCR curves with different threshold cycles can have similar f-values. PCR amplification of the transcript of the tumor necrosis factor (TNF) was conducted on 15 different RNA samples obtained from human testicular tissue. The threshold cycles varied within a range of 3 cycles (24.2 to 26.8). The inset shows the boxplot of the f-values from 15 different amplification curves.