Parameters | Description | Range |
---|---|---|
Consensus Sequence Patterns | ||
Pattern #1 (C/(G/C) | Position -6/-7 | Yes/No |
Pattern #2 (Kozak) | Position -3/+4 | Yes/No |
Consensus Sequence Patterns | ||
5'-UTR Length | Length of 5'-UTR | 80 to 2000 bp |
ORF Length | Length of ORF from the annotated start codon to the stop codon | 350 to 5000 bp |
Start Codon | Frequency of aTIS in training set | 0 to1 |
Number of AUGs | Number of upstream AUGs from aTIS | 0 to 19 |
G/C Ratio | Normalized ratio of G to C | -1 to 1 |
Secondary Structure | ||
Free Energy | 50 bp UnaFold | -40 to 0 |
Secondary Structure | UnaFold | 0 = stem, 3 = loop |