Figure 2
From: Triad pattern algorithm for predicting strong promoter candidates in bacterial genomes
Diagram of the fusion DNA constructs used to express the G. stearothermophilus argC -reporter gene from putative strong promoters of T. maritima in a cell-free system. The argC gene was amplified with forward 5'-GGAGGGGGAACATATGATGAA and reverse 5'-GGACCACCGCGCTACTGCCG primers from pHAV2 [32] by conserving a 112-bp downstream region carrying transcriptional terminators of the vector DNA.