Identification and Annotation of Altered Regions. The Annotate Regions option is an analysis tool that allows you to record regions of interest (i.e. amplifications, deletions), save them to the database, and create ISCN reports. Annotating regions can be used side by side with segmentation probabilities to verify the called regions and can also be used to compare amplification and deletions across multiple samples or create Frequency Plots. Numbers indicate genomic view of (1) annotated regions and (2) segmentation calls, and chromosome view of (3) annotation form where user can mark the region as an amplification, gain, deletion, loss or neutral region and (4) segmentation calls which aid in making the calls.