Problems at the boundaries. Here we see a typical tile (tile 44) superimposed upon a summarization plot. The tile graph was generated using plotTile, and the summarization using plotSummary with summary = 2. The overlap of the two graphs (and the arrow) were produced using R, but are not produced automatically by tileQC. The red dots on the top of the tile indicate reads for which Bustard was unable to make a base-call. The dots in the summarization graph denote the number of reads (per tile) in each of the Eland categories. Note the droop in the blue U0 dots.