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Table 2 Estimated flux ratios from GC-MS measurements of B. subtilis.

From: An analytic and systematic framework for estimating metabolic flux ratios from 13C tracer experiments

flux ratios

our fw (UL)

our fw (1CL)

FiatFlux (UL)

FiatFlux (1CL)

PYR from MAL : PYR from PEP

0.01 : 0.99

 

0.04 : 0.96

 

OAA from TCA-cycle : OAA from PYR

0.42 : 0.58

0.37 : 0.63

0.41 : 0.59

 

PEP from OAA : PEP from GA3P

0 : 1

0 : 1

0.04 :0.96

0 : 1

GLY from SER : GLY from C1 + CO2

1 : 0

1 : 0

1 : 0

 

SER from GLY : SER from GA3P

0.09 : 0.91

 

0.14 : 0.86

 
  1. Directly comparable flux ratios computed from the GC-MS data described by the framework presented in this paper (our fw) and by FiatFlux software [30]. UL denotes uniform labelling of external glucose, 1CL external glucose labelled to the first carbon position.