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Table 3 Percentage of perfectly aligned queries.

From: Scipio: Using protein sequences to determine the precise exon/intron structures of genes and their orthologs in closely related species

   BLAT Exnrt. BLAT Exnrt.
Species Scipio (automatic assembling) with manual assembling without assembling
Bombyx mori p50T 25.0 0.0 25.0 0.0 12.5
Drosophila simulans 37.8 2.7 21.6 2.7 16.2
Bombyx mori str. Dazao 15.6 3.1 12.5 0.0 9.4
Nasonia vitripennis (contigs) 71.4 11.4 57.1 11.4 54.3
Nasonia vitripennis (scaffolds) 68.6 11.4 65.7 11.4 65.7
Drosophila sechellia 63.2 7.9 55.3 2.6 55.3
Aedes aegypti 87.5 7.5 52.5 7.5 50.0
Daphnia pulex 88.1 0.0 76.2 0.0 76.2
Anopheles gambiae 94.6 8.1 73.0 8.1 73.0
Drosophila melanogaster 100.0 10.5 86.8 10.5 86.8
Aspergillus niger 86.7 6.7 73.3 6.7 73.3
Homo sapiens 62.5 0.0 50.0 0.0 50.0
Macaca mulatta 12.5 0.0 10.0 0.0 10.0
total 64.5 5.5 51.7 4.8 50.3
  1. The number of query sequences that were predicted by the programs exactly at the correct location, with 100 % matching residues, without frameshifts or false positives. This figure reveals the amount of workload needed for manual postprocessing of the hits.