TUG expression profile in the early stages of gametophyte development of C. richardii by SCC. (a) Unsupervised two-dimensional hierarchical clustering. Data are presented in a matrix format: each row represents an individual TUG, and each column corresponding to an experimental sample. Each expression measurement represents the normalized log2 ratio of fluorescence from the hybridized experimental sample to a reference sample. Normalized TUG expression ratios are depicted by a pseudocolor scale with red indicating positive expression above the reference, black indicating equal expression as the reference, and green indicating negative expression below the reference. The horizontal colored boxes delimit four pairwise time point comparison groups: 0:24 hr (violet box), 6:24 hr (orange box), 12:24 hr (green box), and 48:24 hr (red box). The scale to the left of the dendrograms depicts the Pearson correlation coefficient represented by the length of the dendrograms branches connecting pairs of nodes. (b) The fold change scale extends from fluorescence ratios of -1 to 1 in log2units. (c) Average expression profiles of Cluster A, computed by averaging the log2(Cy5/Cy3) ratios. (d) Average expression profiles of Cluster B, computed by averaging the log2(Cy5/Cy3) ratios.