Skip to main content
Figure 2 | BMC Bioinformatics

Figure 2

From: A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition

Figure 2

Estimation of PCR efficiency using LinReg method. Efficiency values were determined from 420 independent reactions using a combination of 3.14 × 107–3.14 × 101 DNA molecules as starting template and amplification mix quantities ranging from 60% to 100%. The graph shows the distribution of PCR efficiencies in relation to the percentage of amplification mix used in the reaction. The solid black squares (▪) represent the mean of each distribution.

Back to article page

BMC Bioinformatics

ISSN: 1471-2105

Contact us