Figure 5

Comparison of the Ct , Cp and Cy ₀ methods in terms of precision and accuracy. The accuracy of each method has been reported as Relative Error (RE = expected value – estimated value) while the precision was evaluated measuring the variation coefficient (CV%). The 3D plots show the variation of relative error in relation to amplification mix percentage and log₁₀ input DNA for the Ct (A), Cp (C) and Cy ₀ (E) methods. The areas in the level curve graphs represent the CV% values obtained for each amplification mix percentage and Log₁₀ input DNA combination using the Ct (B), Cp (D) and Cy ₀ (F) methods.