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Table 3 Comparison of mean Relative Error and mean Variation Coefficient among the Ct, Cp, Cy 0 and SCF methods.

From: A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition

 

Ct

Cp

Cy 0

SCF

Log 10 SCF

Mean CV%

39.70%

21.80%

22.52%

594.74%a

66.12%a

Mean RE

-0.318

-0.184

-0.128

-5.058a

-0.205a

  1. The reported data were calculated on 420 PCRs except for a) in which the reaction number was 210.
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BMC Bioinformatics

ISSN: 1471-2105

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