Comparison of multiple alignment tools in execution time for various lengths of the sequences (maximal sequence length, 5000 bases). The relationships between the length of the sequences (maximum, 5000 bases) and the execution time for MXSCARNA, Murlet and Stemloc are plotted. A set of randomly generated sequences of a same length is used for each alignment. The number of the sequences used for the alignment is indicated after the names of the tools. The accuracies for the sequences longer than 500 bases have not been evaluated.