Analysis of the richness and community membership when peptide fragments identified in individual sequence reads were used to assemble the Bacillus anthracis str. Ames genome sequence. (A) The collector's curves for three non-parametric richness estimators and observed richness using individual sequence reads compared to the OPF richness of the assembled genome (horizontal black line). The solid lines represent the richness of non-merged OPFs and the dashed lines represent the richness of merged OPFs with a penalty of 0.15. (B) Collector's curves of parameters describing the similarity between two randomly selected subsets of peptide fragments.