Figure 1From: Large-scale identification of polymorphic microsatellites using an in silico approachAn example of unreliable polymorphic SSRs. Since the repeat chain in EST 3 and 4 does not extend to the end it is not clear whether these two ESTs represent a different (shorter) allele of the SSR or not. For that reason a minimum length for the flanking sequence used must be specified to reliably detect polymorphic SSRs.Back to article page