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Table 1 Number of ESTs, clusters, SSRs and polymorphic SSRs of chicken, rice, Arabidopsis, Brassica, potato and tomato

From: Large-scale identification of polymorphic microsatellites using an in silico approach

 

chicken

rice

Arabidopsis

Brassica

potato

tomato

ESTs

599,330

1,211,078

734,275

163,750

219,765

249,794

Non-redundant sequences1

283,434

493,818

224,994

58,260

72,381

54,182

Clusters1

44,654

35,154

33,052

20,468

25,228

21,229

Singletons1

238,780

458,664

191,942

37,792

47,153

32,953

ESTs with SSRs (%)2

74,297 (12%)

336,569 (28%)

127,757 (17%)

14,968 (9%)

29,481 (13%)

28,728 (11%)

SSRs Non-redundant sequences (%)2

40,020 (14%)

133,861 (27%)

38,096 (17%)

13,251 (23%)

10,537 (15%)

7,163 (13%)

Singletons with SSRs (%)2

31,119 (13%)

118,649 (26%)

29,843 (16%)

7,328 (19%)

5,717 (12%)

3,261 (10%)

SSR in clusters (%)2

8,901 (20%)

15,212 (43%)

8,253 (25%)

5,923 (29%)

4,820 (19%)

3,902 (18%)

Polymorphic SSRs3 (%)

1,724 (19%)

2,646 (17%)

1,248 (15%)

997 (17%)

1,080 (22%)

265 (7%)

Polymorphic SSRs with primers (%)

1,667 (97%)

2,555 (97%)

1,163 (93%)

937 (94%)

1,053 (97%)

263 (99%)

% polymorphism in long SSRs4

15%

11%

12%

13%

17%

6%

% polymorphism in short SSRs5

36%

23%

30%

40%

46%

14%

  1. 1 clusters and singletons were produced using CAP3 with 95% similarity for 100 nucleotides overlaps; 'non-redundant sequences' includes clusters and singletons
  2. 2 SSR detected by Sputnik [13] using default settings
  3. 3polymorphic SSRs detected by PolySSR (settings used are described in Materials and Methods); % = percentage of SSRs in clusters that are polymorphic SSRs.
  4. 4 long polymorphic SSRs are SSRs with at least 10 repeats for dinucleotide SSRs, 6 repeats for trinuleotide SSRs, 5 repeats for tetra-, penta- or hexanucleotide SSRs; % = percentage of polymorphic SSRs in long SSRs;
  5. 5short polymorphic SSRs are SSRs with a maximum of 5 repeats for dinucleotide SSRs, and 4 repeats for tri-, tetra-, penta- and hexanucleotide SSRs; the minimum number of repeats was in this case set to 3 for all SSR types; % = percentage of polymorphic SSR in short SSRs