Figure 2

TIS detection competency tests. Shown are two distinct testing scenarios for TIS identification competency in maximal, TIS-containing reading frames and in reading frames lacking a true TIS. In TIS-containing tests, three outcomes are possible: the system predicts the true TIS as the TIS for the gene (TP), it predicts a false TIS as the gene's TIS (FP), or it fails to predict any TIS for the gene (FN). In the non-TIS-containing scenario, the system either (correctly) refuses to predict a TIS for the gene (TN) or mislabels some in-frame ATG as a TIS (FP).