Figure 2

An easyExon pipeline for analyzing exon array data. (A) Step 1-1, the data input interface. Users have to specify the path of exon-level normalized profile, an optional DABG file (circled in red). (B) Step 2, array grouping. The gene-level summary file is loaded in this step. Users can also specify the array type, meta-probeset profile, exon/gene level of filtration and statistical methods. (C) Step 3, statistical and biological filtration. Exon and gene expression level can be filtered by fold change and/or p-value from the previous selected statistical methods. Users can also search for genes of interest by specifying GO categories or typing in gene IDs. (D) Step 4, annotation. This table implements with a "user-defining" interface for users to select or de-select features. (E) Step 5, graphic presentation of analysis results. This figure includes the probeset intensity plot (upper), SI (Splicing Index) plot (middle) and the exon annotation information (lower). In the exon annotation plot, exons are separated by different colors.