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Table 1 Applied staining methods.

From: An incremental approach to automated protein localisation

cell compartment

dye

cytoplasm including nucleus

GFP without fusion

cytoplasm without nucleus

GFP fused to a cytoplasmic protein

endoplasmic reticulum (ER)

200 nM DiOC5(3)

lysosomes

2 μ M LysoSensor™ Green DND-153

microtubules

1 μ M Oregon Green Paclitaxel

mitochondria

1 μ M MitoTracker® Orange CM-H2TMRos

nucleoli

YFP-Nop56

nucleus

3 μ M Hoechst 33342

peroxisomes

GFP-SKL

plasma membrane

6 μ M FM 1–43

  1. The images depicting several protein localisations were obtained by constructing fusion proteins, while others were acquired using conventional dyes.