From: An incremental approach to automated protein localisation
cell compartment | dye |
---|---|
cytoplasm including nucleus | GFP without fusion |
cytoplasm without nucleus | GFP fused to a cytoplasmic protein |
endoplasmic reticulum (ER) | 200 nM DiOC5(3) |
lysosomes | 2 μ M LysoSensor™ Green DND-153 |
microtubules | 1 μ M Oregon Green Paclitaxel |
mitochondria | 1 μ M MitoTracker® Orange CM-H2TMRos |
nucleoli | YFP-Nop56 |
nucleus | 3 μ M Hoechst 33342 |
peroxisomes | GFP-SKL |
plasma membrane | 6 μ M FM 1–43 |