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Table 1 Statistics of the fluorescent reagent based manual counts for the determination of the reference viability of the mixed cultures

From: A machine vision system for automated non-invasive assessment of cell viability via dark field microscopy, wavelet feature selection and classification

MR*

count 1

count 2

count 3

count 4

count 5

ML**(%)

SD***

 

#live

#dead

#live

#dead

#live

#dead

#live

#dead

#live

#dead

  

1:4

34

163

30

147

24

150

36

144

48

141

18.7

0.044

2:3

65

118

72

116

67

118

80

108

77

127

38.1

0.027

3:2

95

86

93

83

110

65

115

69

101

86

56.9

0.052

4:1

154

39

121

37

120

42

130

52

130

38

75.9

0.032

9:1

143

38

158

37

147

26

131

34

135

39

80.4

0.028

17:3

165

19

130

31

156

22

150

16

142

36

85.6

0.050

  1. * MR: mixture ratio; ** ML: mean percentage of live cells; *** SD: standard deviation