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Table 3 Experimental results of primer pairs designed with QuantPrime

From: QuantPrime – a flexible tool for reliable high-throughput primer design for quantitative PCR

Experiment Predicted gDNA-safe Quality control passed1 Quality control passed1 for detectable transcripts2
A. thaliana 113/128 (88.3%) 117/128 (91.4%) 117/122 (95.9%)
C. reinhardtii 24/33 (72.7%) 28/33 (84.8%) 28/29 (96.6%)
H. vulgare 3 - 27/30 (90.0%) 27/28 (96.4%)
  137/161 (85.1%) 172/191 (90.1%) 172/179 (96.1%)
  1. The primer pairs were designed for wet-lab quantification experiments. The number of primer pairs passing strict quality control checks (melting curve analysis, agarose gel separation and efficiency check) are reported in the table.
  2. 1 Melting curve analysis, gel analysis and efficiency check (E ≥ 1.8) passed. 2 Undetectable transcripts (Ct > 40) removed from the statistics. 3 TIGR Transcript Assembly annotation used, no genomic sequences available.
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