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Table 3 Experimental results of primer pairs designed with QuantPrime

From: QuantPrime – a flexible tool for reliable high-throughput primer design for quantitative PCR

Experiment

Predicted gDNA-safe

Quality control passed1

Quality control passed1 for detectable transcripts2

A. thaliana

113/128 (88.3%)

117/128 (91.4%)

117/122 (95.9%)

C. reinhardtii

24/33 (72.7%)

28/33 (84.8%)

28/29 (96.6%)

H. vulgare 3

-

27/30 (90.0%)

27/28 (96.4%)

 

137/161 (85.1%)

172/191 (90.1%)

172/179 (96.1%)

  1. The primer pairs were designed for wet-lab quantification experiments. The number of primer pairs passing strict quality control checks (melting curve analysis, agarose gel separation and efficiency check) are reported in the table.
  2. 1 Melting curve analysis, gel analysis and efficiency check (E ≥ 1.8) passed. 2 Undetectable transcripts (Ct > 40) removed from the statistics. 3 TIGR Transcript Assembly annotation used, no genomic sequences available.
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BMC Bioinformatics

ISSN: 1471-2105

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