Table 1 Accuracy of scoring the metaphase and telophase phenotype
From: CellProfiler Analyst: data exploration and analysis software for complex image-based screens
| Metaphase | Telophase | |||
|---|---|---|---|---|
| # positive cells (Observer 1) | 35 | 25 | ||
| # positive cells (Observer 2) | 40 | 38 | ||
| Mean # positive cells (Observers 1&2) | 37.5 | 31.5 | ||
| # positive cells (CellProfiler) | 16 | 20 | ||
| # cells called positive by CellProfiler but not by Observers | 0 | 1 | ||
| # cells called positive by Observers but not by CellProfiler | 21.5 | (37.5-16) | 11.5 | (31.5-20) |
| false positive rate | 0% | (0/16) | 5% | (1/20) |
| false negative rate | 57% | (21.5/37.5) | 37% | (11.5/31.5) |