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Table 1 Accuracy of scoring the metaphase and telophase phenotype

From: CellProfiler Analyst: data exploration and analysis software for complex image-based screens

  Metaphase Telophase
# positive cells (Observer 1) 35   25  
# positive cells (Observer 2) 40   38  
Mean # positive cells (Observers 1&2) 37.5   31.5  
# positive cells (CellProfiler) 16   20  
# cells called positive by CellProfiler but not by Observers 0   1  
# cells called positive by Observers but not by CellProfiler 21.5 (37.5-16) 11.5 (31.5-20)
false positive rate 0% (0/16) 5% (1/20)
false negative rate 57% (21.5/37.5) 37% (11.5/31.5)
  1. Ten randomly selected images from the screen (~5000 cells total) were scored by CellProfiler Analyst and by two observers.