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Table 1 Accuracy of scoring the metaphase and telophase phenotype

From: CellProfiler Analyst: data exploration and analysis software for complex image-based screens

 

Metaphase

Telophase

# positive cells (Observer 1)

35

 

25

 

# positive cells (Observer 2)

40

 

38

 

Mean # positive cells (Observers 1&2)

37.5

 

31.5

 

# positive cells (CellProfiler)

16

 

20

 

# cells called positive by CellProfiler but not by Observers

0

 

1

 

# cells called positive by Observers but not by CellProfiler

21.5

(37.5-16)

11.5

(31.5-20)

false positive rate

0%

(0/16)

5%

(1/20)

false negative rate

57%

(21.5/37.5)

37%

(11.5/31.5)

  1. Ten randomly selected images from the screen (~5000 cells total) were scored by CellProfiler Analyst and by two observers.