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Figure 5 | BMC Bioinformatics

Figure 5

From: Position dependent mismatch discrimination on DNA microarrays – experiments and model

Figure 5

Positional influence of single base MM defects on the duplex stability for two different NN pair free energies Δ g ° at a temperature of 310 K. Curves a to f correspond to defect destabilization values δ Δ g d e f MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGaciGaaiaabeqaaeqabiWaaaGcbaGaeqiTdqMaeuiLdqKaem4zaC2aa0baaSqaaiabdsgaKjabdwgaLjabdAgaMbqaaiablIHiVbaaaaa@3597@ of 0 to 5 kcal/mol (incrementally increased by 1 kcal/mol). Defect destabilization δ Δ g d e f MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGaciGaaiaabeqaaeqabiWaaaGcbaGaeqiTdqMaeuiLdqKaem4zaC2aa0baaSqaaiabdsgaKjabdwgaLjabdAgaMbqaaiablIHiVbaaaaa@3597@ is quoted per affected NN pair. (A) Δg° = -1.4 kcal/mol, this corresponds to an average NN-pair free energy; (B) Δg° = -0.8 kcal/mol corresponding to a weakly bound sequence of A·T and T·A base pairs.

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