Figure 3From: The APEX Quantitative Proteomics Tool: Generating protein quantitation estimates from LC-MS/MS proteomics resultsComputing Oi Values. This figure provides an overview of Oi generation. Each protein undergoes an in silico trypsin digestion to form peptide sequences that are fed into a classifier trained on previously constructed training data. The classifier generates peptide detection probabilities. Peptide probabilities related to a particular protein i are summed to arrive at the protein's Oi value, or predicted detected spectral count for one instance of the protein.Back to article page