An Exemplary Analysis Performed with ReplicationDomain. Visual inspection with the aid of ReplicationDomain identifies a region where replication timing has changed during differentiation of ESCs to NPCs. Users can download the data shown on the screen by selecting the "Save Data" option of each data set (A). Downloaded data for each data set will be in a tab-delimited text file with a description at the top (B). Data from multiple differentiation states can be assembled and their differentials calculated in Microsoft Excel (C) and visualized graphically (D). The graph (D) shows a comparison of D3ESC (dark blue) and D3NPC/EBM9 (green) replication timing profiles and their differentials (NPC-ESC; magenta) on chr8:44,000,000–46,000,000.