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Table 2 Conservation of DNA binding residues

From: ProfileGrids as a new visual representation of large multiple sequence alignments: a case study of the RecA protein family

Residue

% Freq.

Other residues

162-Ser

59

Ala 14%, Gln 12%

164-Met

20

Val 62%

165-Gly

99

 

168-Ala

100

 

169-Arg

99

 

172-Ser

99

 

176-Arg

99

 

196-Arg

99

 

197-Met

47

Glu 42%

198-Lys

98

 

199-Ile

74

Val 25%

200-Gly

99

 

207-Glu*

99

 

208-Thr

90

 

211-Gly

100

 

212-Gly

99

 

213-Asn

52

Arg 31%

226-Arg*

97

 

243-Arg*

56

Lys 41%

245-Lys*

96

 

280-Lys*

30

Glu 32%, Asp 16%

282-Lys*

19

Gly 36%, Asp 29%

286-Lys*

93

 

302-Lys*

46

Arg 47%

  1. The first column lists E. coli RecA residues directly involved in DNA binding and those residues proposed (*) to interact with DNA [29]. The "% Freq." column reports the percent frequency of the indicated amino acid among 300 RecA homologs. The last column shows the percent frequency of other residues at that position of the alignment. See text for a description of conservation at these positions among eukaryotic and archaeal RecA homologs.