Figure 4

Sandwich immunoassay design for IA-2. (A) 3D view. Peptide #1 (in red) and peptide #4 (in blue) are represented on the 3D structure of IA-2. (B) Graphic representation. The graph indicates the reactivity of the polyclonal (Abs) with IA-2, in a sandwich-type ELISA format. The Ag, captured by the immobilized Ab, is detected by a biotinylated Ab, followed by addition of streptavidin-peroxidase. Reactivity of×, anti-peptide #4 with IA-2, detected by anti-peptide #1; ▲, anti-peptide #4 with TPO, detected by anti-peptide #1; ■, anti-peptide #15 with IA-2, detected by anti-peptide #1; ◆, anti-peptide #15 with TPO, detected by anti-peptide #1.