Target | Detection | Dilution | EA0 group | F0 arithmetic mean | SEM | Precision relative error (%) | Accuracy factor |
---|---|---|---|---|---|---|---|
SLC6A14 rat | hydrolysis probe | original | 1.91 | 4.1 × 10-8 | 0.06 × 10-8 | 2 | 1.12 (1.22)a |
1 : 4 | 9.0 × 10-9 | 0.4 × 10-9 | 4 | ||||
1 : 16 | 1.7 × 10-9 | 0.3 × 10-9 | 17 | ||||
1 : 64 | 4.6 × 10-10 | 0.2 × 10-10 | 5 | ||||
1 : 256 | 1.0 × 10-10 | 0.2 × 10-10 | 15 | ||||
SLC22A13 human | hydrolysis probe | original | 1.86 | 6.1 × 10-7 | 0.2 × 10-7 | 3 | 0.92 (0.99) |
1 : 4 | 1.7 × 10-7 | 0.07 × 10-7 | 4 | ||||
1 : 16 | 4.0 × 10-8 | 0.08 × 10-8 | 2 | ||||
1 : 64 | 1.2 × 10-8 | 0.05 × 10-8 | 5 | ||||
1 : 256 | 3.3 × 10-9 | 0.02 × 10-9 | 7 | ||||
EMT pig | hydrolysis probe | original | 1.79 | 1.1 × 10-4 | 0.06 × 10-4 | 6 | 1.02 (1.11) |
1 : 4 | 3.4 × 10-5 | 0.2 × 10-5 | 6 | ||||
1 : 16 | 8.4 × 10-6 | 0.4 × 10-6 | 5 | ||||
1 : 64 | 1.7 × 10-6 | 0.5 × 10-6 | 31 | ||||
1 : 256 | 4.0 × 10-7 | 0.2 × 10-7 | 6 | ||||
ETT chicken | SYBR green | original | 1.88 | 1.1 × 10-4 | 0.2 × 10-4 | 16 | 0.97(1.03) |
1 : 4 | 2.3 × 10-5 | 0.5 × 10-5 | 23 | ||||
1 : 16 | 8.1 × 10-6 | 0.6 × 10-6 | 8 | ||||
1 : 64 | 1.9 × 10-6 | 0.07 × 10-6 | 4 | ||||
1 : 256 | 4.9 × 10-7 | 0.2 × 10-7 | 4 | ||||
GAPDH human | SYBR green | original | 1.99 | 1.3 × 10-7 | 0.06 × 10-7 | 5 | 0.99 (1.07) |
1 : 4 | 4.4 × 10-8 | 0.2 × 10-8 | 6 | ||||
1 : 16 | 1.1 × 10-8 | 0.03 × 10-8 | 2 | ||||
1 : 64 | 2.3 × 10-9 | 0.06 × 10-9 | 3 | ||||
1 : 256 | 5.2 × 10-10 | 0.6 × 10-10 | 11 |