Standard curve (Pfaffl) approach | Present paper approach | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Target | Dilution step 1 : 4 | EA subgroup | Factor arithmetic mean | SEM | Precision relative error (%) | Accuracy relative error (%) | EA0 subgroup | Factor arithmetic mean | SEM | Precision relative error (%) | Accuracy relative error (%) |
SLC6A14 rat | 1 | 1.77 | 4.8 | 0.5 | 11 | 21 | 1.92 | 4.5 | 0.2 | 3 | 14 |
2 | 1.74 | 5.0 | 1.4 | 28 | 25 | 1.92 | 5.7 | 1.0 | 17 | 42 | |
3 | 1.80 | 3.1 | 0.9 | 28 | 21 | 1.89 | 3.6 | 0.5 | 14 | 9 | |
4 | 4.7 | 1.3 | 28 | 18 | 4.9 | 1.1 | 23 | 22 | |||
SLC22A13 human | 1 | 2.08 | 2.9 | 0.2 | 7 | 29 | 1.86 | 3.6 | 0.1 | 3 | 10 |
2 | 2.04 | 5.7 | 0.2 | 3 | 43 | 1.86 | 4.3 | 0.1 | 3 | 6 | |
3 | 1.82 | 2.7 | 0.3 | 12 | 33 | 1.86 | 3.3 | 0.2 | 6 | 17 | |
4 | 6.5 | 1.4 | 21 | 61 | 3.7 | 0.4 | 11 | 8 | |||
EMT pig | 1 | 1.71 | 3.1 | 0.1 | 4 | 23 | 1.81 | 3.3 | 0.1 | 2 | 18 |
2 | 1.76 | 2.5 | 0.9 | 35 | 38 | 1.79 | 4.1 | 0.3 | 8 | 3 | |
3 | 1.77 | 9.6 | 4.1 | 42 | 140 | 1.78 | 7.0 | 3.5 | 50 | 75 | |
4 | 5.3 | 1.8 | 33 | 33 | 4.2 | 1.2 | 28 | 5 | |||
ETT chicken | 1 | 1.87 | 6.7 | 2.0 | 30 | 66 | 1.89 | 5.3 | 1.5 | 27 | 33 |
2 | 2.01 | 2.8 | 0.6 | 22 | 31 | 1.89 | 2.9 | 0.7 | 25 | 28 | |
3 | 1.83 | 4.9 | 0.6 | 12 | 21 | 1.86 | 4.3 | 0.3 | 8 | 7 | |
4 | 3.7 | 0.4 | 10 | 7 | 3.9 | 0.2 | 5 | 3 | |||
GAPDH human | 1 | 1.91 | 2.0 | 0.1 | 5 | 50 | 2.00 | 3.0 | 0.1 | 3 | 26 |
2 | 1.92 | 5.0 | 0.3 | 6 | 26 | 1.96 | 4.0 | 0.3 | 7 | 0 | |
3 | 1.84 | 4.9 | 0.1 | 1 | 22 | 1.99 | 4.8 | 0.2 | 5 | 20 | |
4 | 4.3 | 0.1 | 2 | 6 | 4.5 | 0.4 | 9 | 12 | |||
Median: | 12 | 27.5 | Median: | 8 | 13 |