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Table 4 Analysis with 2 previous approaches that work without standard curves. Data for table 2 was analyzed as 3 subgroups of 5 samples each. For each curve, fluorescence of point 10 was subtracted as background from all points. With the DART-PCR approach, each curve was first analyzed separately for EA. R0 values (that correspond to F0) were calculated with the average EA for each subgroup. With the LinRegPCR approach, software version 7.2 was used to analyze each curve separately.

From: Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence

  

DART-PCR approach

LinRegPCR approach

Target

Dilution step 1 : 4

mean EA subgroup

Factor arithmetic mean

SEM

Precision relative error (%)

Accuracy relative error (%)

EA range

Factor arithmetic mean

SEM

Precision relative error (%)

Accuracy relative error (%)

SLC6A14 rat

1

1.97

4.7

0.2

5

18

1.79 – 2.04

71.7

57

79

>1000

 

2

1.94

6.4

1.6

25

61

1.71 – 2.09

0.94

0.23

24

77

 

3

1.86

3.6

0.5

14

10

1.83 – 2.79

>1000

>1000

100

>1000

 

4

 

5.0

1.1

21

26

 

8.6

8.5

99

114

SLC22A13 human

1

1.70

3.2

0.1

3

21

1.64 – 2.01

6.4

2.9

45

59

 

2

1.70

3.3

0.1

2

17

1.68 – 2.24

254

227

89

>1000

 

3

1.71

3.0

0.1

1

25

1.52 – 1.93

412

412

100

>1000

 

4

 

3.1

0.1

4

23

 

36

36

100

797

EMT pig

1

 

3.0

0.2

6

25

1.67 – 2.02

8.8

7.7

87

121

 

2

1.78

3.7

0.2

6

7

1.66 – 1.84

7.2

3.5

49

80

 

3

1.87

6.8

2.9

43

69

1.60 – 1.75

11.7

4.9

42

193

 

4

1.70

4.2

1.2

29

4

 

3.4

0.9

25

16

ETT chicken

1

1.87

4.9

1.2

25

22

1.77 – 1.91

3.0

0.6

19

24

 

2

1.86

3.0

0.8

26

25

1.76 – 1.88

2.5

0.4

18

37

 

3

1.92

4.0

0.3

7

1

1.85 – 1.92

2.4

0.8

32

39

 

4

 

4.0

0.3

7

0

 

6.4

0.7

12

59

GAPDH human

1

1.86

2.9

0.1

3

28

1.84 – 1.90

4.6

0.4

10

15

 

2

1.84

3.4

0.3

8

15

1.78 – 1.87

3.0

0.8

26

25

 

3

1.81

4.0

0.2

4

1

1.77 – 1.86

6.3

0.5

7

58

 

4

 

3.8

0.3

7

6

 

2.8

1.0

35

29

    

Median:

7

19.5

  

Median:

38.5

58.5