DART-PCR approach | LinRegPCR approach | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Target | Dilution step 1 : 4 | mean EA subgroup | Factor arithmetic mean | SEM | Precision relative error (%) | Accuracy relative error (%) | EA range | Factor arithmetic mean | SEM | Precision relative error (%) | Accuracy relative error (%) |
SLC6A14 rat | 1 | 1.97 | 4.7 | 0.2 | 5 | 18 | 1.79 – 2.04 | 71.7 | 57 | 79 | >1000 |
2 | 1.94 | 6.4 | 1.6 | 25 | 61 | 1.71 – 2.09 | 0.94 | 0.23 | 24 | 77 | |
3 | 1.86 | 3.6 | 0.5 | 14 | 10 | 1.83 – 2.79 | >1000 | >1000 | 100 | >1000 | |
4 | 5.0 | 1.1 | 21 | 26 | 8.6 | 8.5 | 99 | 114 | |||
SLC22A13 human | 1 | 1.70 | 3.2 | 0.1 | 3 | 21 | 1.64 – 2.01 | 6.4 | 2.9 | 45 | 59 |
2 | 1.70 | 3.3 | 0.1 | 2 | 17 | 1.68 – 2.24 | 254 | 227 | 89 | >1000 | |
3 | 1.71 | 3.0 | 0.1 | 1 | 25 | 1.52 – 1.93 | 412 | 412 | 100 | >1000 | |
4 | 3.1 | 0.1 | 4 | 23 | 36 | 36 | 100 | 797 | |||
EMT pig | 1 | 3.0 | 0.2 | 6 | 25 | 1.67 – 2.02 | 8.8 | 7.7 | 87 | 121 | |
2 | 1.78 | 3.7 | 0.2 | 6 | 7 | 1.66 – 1.84 | 7.2 | 3.5 | 49 | 80 | |
3 | 1.87 | 6.8 | 2.9 | 43 | 69 | 1.60 – 1.75 | 11.7 | 4.9 | 42 | 193 | |
4 | 1.70 | 4.2 | 1.2 | 29 | 4 | 3.4 | 0.9 | 25 | 16 | ||
ETT chicken | 1 | 1.87 | 4.9 | 1.2 | 25 | 22 | 1.77 – 1.91 | 3.0 | 0.6 | 19 | 24 |
2 | 1.86 | 3.0 | 0.8 | 26 | 25 | 1.76 – 1.88 | 2.5 | 0.4 | 18 | 37 | |
3 | 1.92 | 4.0 | 0.3 | 7 | 1 | 1.85 – 1.92 | 2.4 | 0.8 | 32 | 39 | |
4 | 4.0 | 0.3 | 7 | 0 | 6.4 | 0.7 | 12 | 59 | |||
GAPDH human | 1 | 1.86 | 2.9 | 0.1 | 3 | 28 | 1.84 – 1.90 | 4.6 | 0.4 | 10 | 15 |
2 | 1.84 | 3.4 | 0.3 | 8 | 15 | 1.78 – 1.87 | 3.0 | 0.8 | 26 | 25 | |
3 | 1.81 | 4.0 | 0.2 | 4 | 1 | 1.77 – 1.86 | 6.3 | 0.5 | 7 | 58 | |
4 | 3.8 | 0.3 | 7 | 6 | 2.8 | 1.0 | 35 | 29 | |||
Median: | 7 | 19.5 | Median: | 38.5 | 58.5 |