Simple genome annotation pipeline. Example of a simple computational pipeline for genome annotation. Green boxes represent nodes. The pipeline describes the execution of a gene predictor (node 3) and two BLAST analyses (nodes 4 and 5)  on a set of input DNA sequences (node 1). The BLAST analysis in node 4 compares the incoming sequences against the NCBI NT database. Node 5 uses a BLAST database created by node 2 from the same set of sequences (self-BLAST).