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Table 2 Identification and analysis of secreted proteins from Trichostrongylus vitrinus ESTs.

From: In silico analysis of expressed sequence tags from Trichostrongylus vitrinus (Nematoda): comparison of the automated ESTExplorer workflow platform with conventional database searches

Number

EST sequence ID

Seq Length (aa)

Start

Signal Peptide length (aa)

Description (top hit from non-redundant protein database)

E-value

% Identity (aa)

RNAi phenotype in C. elegans

1

TvmContig8

102

-

19

No significant hits

-

-

-

2

TVmContig11

148

-

24

No significant hits

-

-

-

3

TVmContig20

121

-

25

24 kDa excretory/secretory protein [Haemonchus contortus]

3.00E-028

63/109 (57%)

 

4

TvmContig28

167

-

19

No significant hits

-

-

-

5

TVmContig35

120

M

17

30 kDa antigenic glycoprotein precursor [Trichostrongylus colubriformis]

4.00E-006

27/65 (41%)

No observed phenotype is found.

6

TVmContig39

208

-

18

Weakly similar to PhosphoDiEsterase family member (pde-2) [Caenorhabditis elegans]

4.00E-028

22/72 (30%)

No observed phenotype is found.

7

TvmContig68

131

-

26

Weakly similar to SaPosin-like Protein family member (spp-19) [Caenorhabditis elegans]

0.016

23/82 (28%)

No observed phenotype is found.

8

TVmContig75

239

-

26

Weakly similar to Na/Ca eXchangers family member (ncx-4) [Caenorhabditis elegans]

0.3

15/39 (38%)

No observed phenotype is found.

9

TVmContig96

120

-

15

Non-ATPase-like family member (rpn-1) [Caenorhabditis elegans]

2.00E-047

94/119 (78%)

EMB, LET embryonic_lethal, maternal_sterile, sick (Sck)

10

TvmContig101

156

M

18

Zinc metalloproteinase nas-13 precursor (Nematode astacin 13) [Caenorhabditis elegans]

2.00E-008

30/96 (31%)

No observed phenotype is found.

11

TVmContig107

85

-

27

No significant hits

-

-

-

12

TVmContig134

190

-

26

No significant hits

-

-

-

13

TvmContig135

116

-

19

Late Embryo Abundant (LEA) related family member (lea-1) [Caenorhabditis elegans]

4.00E-004

22/86 (25%)

No observed phenotype is found.

14

TVmContig150

137

-

24

No significant hits

-

-

-

15

TVmContig164

122

-

20

Weakly similar to Human xnp gene related protein 1 [Caenorhabditis elegans]

0.18

18/57 (31%)

None

16

TVmContig170

85

M

19

No significant hits

-

-

-

17

TVmContig171

131

M

15

No significant hits

-

-

-

18

TVmContig172

129

-

26

Weakly similar to abnormal NUClease family member (nuc-1) [Caenorhabditis elegans]

0.016

12/30 (40%)

life span abnormal (Age)

19

TvmContig178

146

-

17

Weakly similar to Maternal Effect Sterile family member (mes-3) [Caenorhabditis elegans]

0.047

13/26 (50%)

STP sterile_progeny

20

TVm01_A12

91

-

18

No significant hits

-

-

-

21

TVm02_D11

86

M

24

No significant hits

-

-

-

22

TVm03_G11

108

-

20

No significant hits

-

-

-

23

TVm04_F02

97

-

19

No significant hits

-

-

-

24

TVm06_F08

72

-

25

No significant hits

-

-

-

25

TVm07_A07

78

-

26

No significant hits

-

-

-

26

TVm07_G02

124

-

16

putative calcium-binding mitochondrial carrier protein [Brugia malayi]

1.00E-036

75/116 (64%)

GRO slow_growth |Unclassified

27

TVm08_D01

64

M

19

Hypothetical protein MGG_00752 [Magnaporthe grisea 70-15]

8.00E-006

25/52 (48%)

None

28

TVm08_H01

120

-

19

Pyruvate kinase [Caenorhabditis elegans]

6.00E-025

42/87 (48%)

EMB, LET embryonic_lethal

29

TvfContig1

152

-

22

No significant hits

-

-

-

30

TvfContig3

143

-

23

C-type LECtin family member (clec-88) [Caenorhabditis elegans]

5.00E-021

55/132 (41%)

No observed phenotype is found.

31

TvfContig5

134

M

22

Hypothetical protein K06A9.1c [Caenorhabditis elegans]

0.004

28/98 (28%)

None

32

TvfContig78

161

-

20

MSH (MutS Homolog) family member (msh-2) [Caenorhabditis elegans]

2.00E-027

70/167 (41%)

spontaneous mutation rate increased

33

TvfContig84

104

M

18

No significant hits

-

-

-

34

TvfContig88

145

M

22

similar to TPRXL protein [Pan troglodytes]

0.002

31/122 (25%)

None

35

TvfContig122

97

-

24

No significant hits

-

-

-

36

TvfContig132

214

M

13

Vitellogenin-6 precursor [Caenorhabditis elegans]

  

GRO slow_growth |EMB, LET embryonic_lethal

37

TVf03_C08

132

-

20

No significant hits

-

-

-

38

TVf04_D01

162

-

16

putative serine-threonine kinase PAR-4 [Caenorhabditis elegans]

0.027

16/35 (45%)

No observed phenotype is found.

39

TVf06_A07

145

-

22

15 kDa excretory/secretory protein [Haemonchus contortus]

6.00E-004

39/118 (33%)

No observed phenotype is found.

40

TVf07_A05

146

-

23

No significant hits

-

-

-