Table 3 Comparision of annotations for T. vitrinus ESTs: EST annotations performed by Nisbet and Gasser [4] based on BLAST similarity results in first three columns and annotations obtained from ESTExplorer are shown in next columns. Example catagories selected for male ESTs are major sperm protein-like, protein kinases and protein phosphatases, transcription factors and related and nucleic acid synthesis and function and for female ESTs, vitellogenins, carbohydrate metabolism and modification, ubiquitin-proteasome pathway, protein kinases/phosphatases and transcription factors and related proteins

MALE: Protein kinases and protein phosphatases

TVm02_C07

2.00E-37

PP1-gamma serine/threonine protein phosphatase

protein phosphatasecatalyticgamma isoform isoform 1

1.00E-36

chromatin modification, protein amino acid dephosphorylation, embryonic cleavage, cytokinesis, meiosis, oviposition, manganese ion binding, protein phosphatase type 1 activity, mitochondrial outer membrane, protein binding, mitosis, glycogen metabolic process, iron ion binding, nucleus

Long-term potentiation, Regulation of actin cytoskeleton, Focal adhesion, Insulin signaling pathway

Metallophosphoesterase, Serine/threonine-specific protein phosphatase and bis(5-nucleosyl)-tetraphosphatase

TVm02_F11

2.00E-45

Serine/threonine protein kinase of the casein kinase I subfamily

tau tubulin kinase

1.00E-44

protein kinase activity, ATP binding, protein amino acid phosphorylation, locomotory behavior, growth, larval development (sensu Nematoda)

Nicotinate and nicotinamide metabolism, Inositol phosphate metabolism, Benzoate degradation via CoA ligation

Protein kinase

TVm08_H06

5.00E-60

Member of the protein phosphatase protein family

serine threonine protein phosphatase pp1

1.00E-64

phosphoprotein phosphatase activity, protein amino acid dephosphorylation, iron ion binding, manganese ion binding

None

Metallophosphoesterase, Serine/threonine-specific protein phosphatase and bis(5-nucleosyl)-tetraphosphatase

TVm09_B11

8.00E-34

Protein phosphatase-1 (PP1)

phosphoprotein phosphatase 1

1.00E-33

growth, larval development (sensu Nematoda), embryonic development ending in birth or egg hatching, hydrolase activity

None

Metallophosphoesterase, Serine/threonine-specific protein phosphatase and bis(5-nucleosyl)-tetraphosphatase

MALE: Transcription factors and related

TVm01_F11

8.00E-10

Transcription elongation factor (testes specific homologue in mouse)

transcription elongation factor a1

1.00E-19

regulation of transcription, DNA-dependent, RNA elongation, translation elongation factor activity, zinc ion binding, RNA polymerase II transcription factor activity, nucleus, DNA binding, defense response, transcription regulator activity, transcription, regulation of transcription, transcription factor activity, transcription elongation factor complex, protein binding, transcriptional elongation regulator activity, erythrocyte differentiation, positive regulation of transcription, DNA-dependent, general RNA polymerase II transcription factor activity, metal ion binding, nucleoplasm

None

None

TVm09_B03

1.00E-09

Protein containing six kelch motifs and one BTB/POZ domain

klhl10 protein

1.00E-10

larval development (sensu Nematoda), growth, protein binding

None

Kelch repeat

FEMALE: Vitellogenins

TVf01_C08

4.00E-25

Vitellogenin 6 precursor

vitellogenin structural genes (yolk protein genes) family member (vit-6)

1.00E-24

embryonic development ending in birth or egg hatching;P:determination of adult life span, lipid transporter activity

None

Lipid transport protein, N-terminal

TVf01_C11

7.00E-14

Vitellogenin 5 precursor

vitellogenin structural genes (yolk protein genes) family member (vit-6)

1.00E-25

embryonic development ending in birth or egg hatching;P:determination of adult life span, lipid transporter activity

None

Lipid transport protein, N-terminal

FEMALE: Carbohydrate metabolism and modification

TVf02_G06

7.70E-15

glyceraldehyde 3 phosphate dehydrogenase gpd-3C

glyceraldehyde-3-phosphate dehydrogenase

1.00E-18

cell wall chitin biosynthetic process, glutamine-fructose-6-phosphate transaminase (isomerizing) activity

None

Glyceraldehyde 3-phosphate dehydrogenase, Lipocalin

TVf02_H12

6.80E-24

glucosamine-fructose-6-phosphate aminotransferase

glutamine-fructose-6-phosphate transaminase 2

1.00E-32

fructose 6-phosphate metabolic process, glutamine-fructose-6-phosphate transaminase (isomerizing) activity

Aminosugars metabolism, Glutamate metabolism

Glutamine amidotransferase, class-II

TVf03_B07

5.80E-28

Pyruvate dehydrogenase alpha subunit

pyruvate dehydrogenase e1 alpha subunit

1.00E-62

oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor, metabolic process, C:mitochondrion

Pyruvate metabolism, Glycolysis/Gluconeogenesis, Valine leucine and isoleucine biosynthesis, Butanoate metabolism

Dehydrogenase, E1 component

TVf03_E01

2.80E-35

Pyruvate dehydrogenase

dihydrolipoamide acetyltransferase

1.00E-53

oxidoreductase activity

Pyruvate metabolism, Glycolysis/Gluconeogenesis, Valine leucine and isoleucine biosynthesis, Butanoate metabolism

Transketolase, central region

TVf06_E11

3.80E-49

gei-7 isocitrate lyase

malate synthase a

1.00E-50

embryonic development, F:acyltransferase activity, tricarboxylic acid cycle, isocitrate lyase activity, determination of adult life span, glyoxylate cycle

Pyruvate metabolism, Glyoxylate and dicarboxylate metabolism

Malate synthase

TVf09_D01

3.20E-08

Ribulose-phosphate 3 epimerase

ribulose-phosphate 3-epimerase

1.00E-17

metabolic process, catalytic activity

Pentose phosphate pathway

Ribulose-phosphate 3-epimerase

FEMALE: Ubiquitin-proteasome pathway

TVf02_H07

3.00E-28

Ubiquitin-like

ubiquitin family member (ubq-1)

1.00E-27

ribosome, larval development (sensu Nematoda), structural constituent of ribosome, reproduction, growth translation, protein modification process

Ribosome

Ubiquitin

TVf06_D11

1.30E-12

pbs-2 Proteasome A-type and B-type

20s proteasome beta subunit pbb2

1.00E-22

threonine endopeptidase activity, ubiquitin-dependent protein catabolism, proteasome core complex (sensu Eukaryota)

Proteasome

20S proteasome, A and B subunits

TVf10_F06

5.20E-07

ubiquitin activating enzyme related (uba-1)

ubiquitin-activating enzyme e1

1.00E-12

ATP binding, ubiquitin-protein ligase activity, ubiquitin activating enzyme activity

Ubiquitin mediated proteolysis

Ubiquitin-activating enzyme repeat

FEMALE: Protein kinases/phosphatases

TVf06_F11

1.40E-49

lin-2 erythrocyte membrane like protein

calcium calmodulin-dependent serine protein kinase (maguk family)

1.00E-51

synapse, guanylate kinase activity, cell adhesion, positive regulation of vulval development (sensu Nematoda), actin cytoskeleton, protein-tyrosine kinase activity, synaptosome, nucleotide binding, basolateral plasma membrane, protein amino acid phosphorylation, cytosol, cell junction, calmodulin binding, protein serine/threonine kinase activity, positive regulation of transcription from RNA polymerase II promoter

Tight junction, Neurodegenerative Disorders

Pkinase_Tyr, Guanylate_kin

FEMALE: Transcription factors and related

TVf01_C02

3.80E-18

ama-1 RNA polymerase II

rna polymerase ii largest subunit

1.00E-30

DNA-directed RNA polymerase activity, DNA binding, transcription from RNA polymerase II promoter, DNA-directed RNA polymerase II, core complex

Purine metabolism, Pyrimidine metabolism, RNA polymerase

RNA polymerase Rpb1, domain 1

TVf03_A03

9.70E-25

btf-1 helicase

tbp associated factor

1.00E-63

nucleic acid binding, helicase activity, ATP binding

Purine metabolism

Helicase, C-terminal

TVf04_E07

2.00E-09

ceh-5 Homeobox domain

similarity to lim-homeobox protein lmx1_mesau

1.00E-15

metal ion binding, sequence-specific DNA binding, transcription factor activity, regulation of transcription, multicellular organismal development DNA-dependent, nucleus

None

None