Figure 3

Calculation of vicinity peak intensity ratio of the base-call position (arrowed). [2]-vicinity ratio (k = 2) is calculated by normalizing the surrounding signal intensities of two bases left and right of the observed position as described in Equation 2 as follows: $Q_v^3$ = 1/4 × (1 + 0.94 + 1 + 0.97) = 0.977