Step-like calcium uncaging. Simulation results showing vesicle release following a step-like Ca2+ uncaging. From left to right are reported the time course of Ca2+, the logarithmic scale of it, showing the intermediate states of calcium binding, and a focus on vesicle activation (V star) and the cumulative amount of vesicle released (T). It can be observed that the pool of vesicles is almost completely depleted within 3 ms. The following set of parameters were utilised: V=100; Ca2+=6000; C
=9500; γ=6000; b=0.25.