Wave-like calcium uncaging. Simulation results showing vesicle release following a wave-like Ca2+ uncaging. From left to right are reported the time course of the Ca2+ wave, the logarithmic scale of it, showing the intermediate states of calcium binding, and a focus on vesicle activation (V star) and the cumulative amount of vesicle released (T). It can be observed that the calcium wave lasts about 1 ms with a half width of about 0.5 ms and a peak value of about 6000, which corresponds to a peak calcium concentration of about 20 μ M. The right side shows the release of one vesicle. The parameters were the same set utilised in the step-like case (see legend of Figure 2). The parameters of the equations describing calcium extrusion were: c1 = 8 s−l, c2 = 25 s−l and c3 = 10000 s−1.