Impact of T-RFLP data analysis choices on assessments of microbial community structure and dynamics

BMC Bioinformatics

Table 1 Number of T-RFs and Jaccard and Bray-Curtis similarities of the loading duplicates dataset after a PDT of 50 and normalization

Treatment	NT-heights	NT-areas	TFN-heights	TFN-areas	TFN-areas-LT	FPT 1% -heights	FPT 1% -areas
No of T-RFs	43 ± 15	43 ± 15	18 ± 8*	42 ± 15	42 ± 15	21 ± 7*	22 ± 7*
Jaccard	71 ± 8%	71 ± 8%	84 ± 11%*	71 ± 8%	71 ± 8%	78 ± 14%*	74 ± 13%
Bray-Curtis	89 ± 6%	87 ± 6%	96 ± 4%**	93 ± 3%*	87 ± 6%	91 ± 8%	88 ± 7%

All values are average values ± the standard deviation. The number of T-RFs is the average for all 102 profiles. The Jaccard and Bray-Curtis similarities are the averages similarities of all 51 duplicate pairs. The T-RF profiles were analyzed with a PDT of 50. Descriptions of the treatments can be found in the Methods section. *denotes a significant difference from NT-heights. **denotes a significant difference from all other treatments.

ISSN: 1471-2105