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Table 5 Correlations between T-RF abundances and WWTP process parameters

From: Impact of T-RFLP data analysis choices on assessments of microbial community structure and dynamics

T-RF size Treatment COD P F/M I H T Fe EtOH PD S SC ESS
167 A    +          
167 B        +      -
167 C             
168 A + +    +       + +
168 B    +   + - -     + +
168 C +   +   + -      + +
303 A             
303 B     +     + +    
303 C     +     + +    
304 A           +   
304 B     -     -   +   
304 C     -     -     
  1. Only correlations significant at the 95% level are shown. + indicate positive correlation and – a negative correlation. Treatment A: PDT 50, No alignment correction, no normalization. Treatment B: PDT 50, Alignment correction using the systematic shift correction procedure, normalization of consensus profiles using the TFN-areas procedure. Treatment C: PDT 100, Alignment correction using the systematic shift correction procedure, normalization of both replicate and consensus profiles using the TFN-heights procedure. In all treatments consensus profiles were generated from two replicate profiles only considering T-RFs present in both. Process parameters: COD - Total COD going into the activated sludge tanks (mg/l), P - Total phosphorus concentration in primary settled wastewater (mg/l), F/M - Food to microorganisms ratio (g/kg*s), I - Inorganic fraction of the activated sludge (%), H - Humic substances in the activated sludge (mg/gMLSS), T - Water temperature, Fe - Total iron dosage, EtOH - Ethanol dosage, PD - Polymer dosage, S - Shear sensitivity of activated sludge flocs, SC - Carbohydrate content in the activated sludge (mg/gMLSS), ESS - Effluent suspended solids (mg/l).