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Table 5 Correlations between T-RF abundances and WWTP process parameters

From: Impact of T-RFLP data analysis choices on assessments of microbial community structure and dynamics

T-RF size

Treatment

COD

P

F/M

I

H

T

Fe

EtOH

PD

S

SC

ESS

167

A

  

+

         

167

B

      

+

    

-

167

C

            

168

A

+

+

  

+

     

+

+

168

B

  

+

 

+

-

-

   

+

+

168

C

+

 

+

 

+

-

    

+

+

303

A

            

303

B

   

+

   

+

+

   

303

C

   

+

   

+

+

   

304

A

         

+

  

304

B

   

-

   

-

 

+

  

304

C

   

-

   

-

    
  1. Only correlations significant at the 95% level are shown. + indicate positive correlation and – a negative correlation. Treatment A: PDT 50, No alignment correction, no normalization. Treatment B: PDT 50, Alignment correction using the systematic shift correction procedure, normalization of consensus profiles using the TFN-areas procedure. Treatment C: PDT 100, Alignment correction using the systematic shift correction procedure, normalization of both replicate and consensus profiles using the TFN-heights procedure. In all treatments consensus profiles were generated from two replicate profiles only considering T-RFs present in both. Process parameters: COD - Total COD going into the activated sludge tanks (mg/l), P - Total phosphorus concentration in primary settled wastewater (mg/l), F/M - Food to microorganisms ratio (g/kg*s), I - Inorganic fraction of the activated sludge (%), H - Humic substances in the activated sludge (mg/gMLSS), T - Water temperature, Fe - Total iron dosage, EtOH - Ethanol dosage, PD - Polymer dosage, S - Shear sensitivity of activated sludge flocs, SC - Carbohydrate content in the activated sludge (mg/gMLSS), ESS - Effluent suspended solids (mg/l).