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Table 2 Required input parameters with example values

From: Tools for T-RFLP data analysis using Excel

Parameter

Value

1. Total number of profiles

6

2. Number of samples

3

3. Number of replicates per sample

2

4. Number of T-RFs

3

5. Sample names on row

1

6. Consider T-RF if present in X replicates. X=

2

7. Align T-RFs with a size difference shorter than Y bases. Y=

1

8. Add T-RFs less than Z bases longer than the average size of the alignment. Z=

0.5

9. Peak height detection threshold. PDT =

50

10. Lower T-RF Size Limit

50

11. Upper T-RF Size Limit

1020

12. Fixed percentage threshold. FPT=

0.01

  1. Parameters 1-5 and 9-11 are used for the initial preparation of the data. Parameters 1-4 are also used by procedures that need the number of samples or replicates to be specified. Parameter 6 is used for the generation of consensus profiles. Parameters 7 and 8 are used in the alignment of T-RF profiles. Parameter 12 is used for normalization with the fixed percentage threshold procedure.