Tools for T-RFLP data analysis using Excel

BMC Bioinformatics

Table 7 Examples of correct alignments that are classified as ambiguous

Samples	1	2	3	4	No of T-RFs	Min size	Max size	Mean size	Ambiguous
T-RF 1	162.99	163.30	163.32	163.03	4	162.99	163.32	163.16	Yes
T-RF 2	164.10	164.88	164.65	164.28	4	164.10	164.88	164.48	Yes
T-RF 3	166.18	0	0	166.95	2	166.18	166.95	166.57	Yes
T-RF 4	167.97	168.25	168.22	167.77	4	167.77	168.25	168.05	Yes

The table replicates the output of the procedure “J_Check_AlignedConsensus”. Following the columns of the six samples, where the given numbers are the T-RF sizes in bases, are columns for the number of T-RFs, the minimum T-RF size, the maximum T-RF size and the mean T-RF size in each alignment bin. The last column is either “Yes” or “No”, indicating whether the alignment bin is ambiguous or not.

ISSN: 1471-2105