Heat map of TSS and FSS. Heat maps of (a) TSS and (b) FSS were generated by using corresponding association matrices. Association matrices were generated by taking 20 positions (10 positions at the exon end and 10 positions excluding GT at the intron start). Since each position corresponds to four indicator variables, hence the heat map generated is of order 80 × 80 units and the units between 29–40 indicates 3 bp at the exon end and 41–64 units for 6 bp at the intron start. There exist distinct association pattern among the positions around the conserved di-nucleotide GT in TSS. On the other hand, such association pattern is absent in case of FSS.