Figure 2From: A fixed-point algorithm for estimating amplification efficiency from a polymerase chain reaction dilution seriesCorrelation between reference curve and data. The correlation between the reference curve and the data of Figure 1 maximizes at a value x 0≈29. The value of the fractional cycle maximizing correlation can be found either by fitting a quadratic through 29 and the adjacent points, or by a one-dimensional non-linear search. For the illustrated data, the maximum is at 28.93 cycles.Back to article page