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Figure 3 | BMC Bioinformatics

Figure 3

From: A fixed-point algorithm for estimating amplification efficiency from a polymerase chain reaction dilution series

Figure 3

The regression of C q against logarithm of dilution (base2). If each PCR cycle were to double the DNA, then a two-fold dilution would delay the curve by one cycle. The slope of the straight line regressing C q against log of dilution (base 2) tells us how many cycles are required to double the DNA. Using a reference curve with A=1.5 slope is 1.185 cycles per doubling, or equivalently increasing the DNA 1.795 - fold per cycle. The next fixed-point iteration will use A=1.795 in the reference curve.

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BMC Bioinformatics

ISSN: 1471-2105

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