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Figure 6 | BMC Bioinformatics

Figure 6

From: A fixed-point algorithm for estimating amplification efficiency from a polymerase chain reaction dilution series

Figure 6

Regression of C q against logarithm of dilution. Amplification efficiency is estimated from the slope of the line relating C q to the dilution, the latter on a logarithmic scale. The confidence limits for the slope, when calculated from unweighted least-squares (as commonly done in simple linear regression) is strictly appropriate only for normally-distributed homoscedastic data. We caution that our data are not homoscedastic, and are unlikely to be normally distributed.

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