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Figure 3 | BMC Bioinformatics

Figure 3

From: Computational approach for calculating the probability of eukaryotic translation initiation from ribo-seq data that takes into account leaky scanning

Figure 3

Comparison of methods for discriminating the strength of AUG TISs from non-AUG TISs in human. A. Probability density plots for human TISs depending on their initiation strength (gray AUG, orange CUG). Left: The scores are calculated as a fraction of the footprints aligning to the TISs from the total number of footprints aligning to the corresponding mRNA using the PAS method (equation (1)). Right: The translation initiation probability scores are calculated using the LS method (equation (4)). Transcripts with two TISs without an in-frame stop codon between the first TIS and second TIS were used. B. Probability density plots for human TISs from all mRNAs (left: PAS method, equation (1); right: LS method, equation (4)). All data are from Lee et al. [4] (Human) and a 3′ artificial start value of Ru = 0.05 RLTM-RCHX was used for the LS method; see Additional file 1: Figure S8 for the other datasets.

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BMC Bioinformatics

ISSN: 1471-2105

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