Novel involvement of HAP2, ERG28 and LYS12 in translation. A) The relative β-galactosidase activity is determined by normalizing the activity of the mutant strains carrying different stop-codon read through cassettes to the control construct (pUKC815 with no premature stop codon) in the wild type strain. B) The relative mRNA level is determined by normalizing the mRNA content of the mutant strains carrying different premature stop-codon expression cassettes to those in the wild type. C) Increased sensitivity of hap2Δ, erg28Δ and lys12Δ to different translation inhibitory drugs. Sensitivity of the wild type strain was used as a point of reference. Sensitivity was quantified as low, moderate and high with respect to that for the wild type strain. hap2Δ, lys12Δ, and erg28Δ show increased sensitivity to one or both streptomycin and/or cycloheximide. D) Effect of gene deletions on translation efficiency. Relative translation efficiency was measured using p416 plasmid containing Gal-inducible promoter in LacZ expression cassette normalized to mRNA content. Values are related to translation efficiency of the control strain set at 1.0.