Transcript reconstruction on simulated reads. (A) Lengths of all transcripts constructed by VTBuilder and Trinity compared to those of the SSTs. The top and bottom of the boxes represent the 25th and 75th percentiles respectively, while the top and bottom whiskers represent the third quartile +1.5 times the inter quartile range (IQR) and the first quartile - 1.5 times the IQR respectively. Outliers beyond these points are represented as black circles. (B) Lengths of transcripts constructed by VTBuilder and Trinity that had a sequence similarity of 90% or greater to the SSTs. (C) Network showing the relationship between the VTBuilder transcripts and the SSTs. Grey nodes represent the VTBuilder transcripts. Colored nodes represent the protein families to which the individual SSTs belong (see key). Node size is proportional to sequence length. Edges represent a 90% or greater sequence similarity. (D) Same as (C) but using Trinity to construct the transcripts.