Relationships between sensitivity and specificity of BLAST, BLAT, and CLAST by changing the identity threshold both of the 100 base test and the 800 base test. Each curve represents the results of simulated metagenomic analysis of BLAST, BLAT, and CLAST (both global and local modes) under several thresholds. Each curve consists of the 5 points, indicating the results of simulated metagenomic analysis with 5 different thresholds. One point is the result that was not filtered by any identity and coverage thresholds (same with the point in Figure 9), and the others are based on the results that were filtered by an identity threshold and a coverage threshold. The identity thresholds were 95%, 90%, 85%, and 80%. The coverage threshold was unified to 50%. In all curves, high identity thresholds represent small numbers of total reported hits and correct genus assignments (Additional file 7). The points of Bowtie 2 results (both global and local modes) that were not filtered by any identity and coverage thresholds (same with the points in Figure 9) are also plotted to be able to compare with the curves of other tools. Horizontal axis represents total reported hits, and vertical axis represents correct genus assignments. (A) Results of 100 base test. (B) Results of 800 base test.